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Worthington胶原酶,现货促销
发布时间:2013-06-19        浏览次数:403        返回列表
 
产品名称 英文名 货号 规格
胶原酶,纯品 Collagenase, Purified LS005275 4 ku
胶原酶,纯品 Collagenase, Purified LS005273 10 ku
胶原酶,I型 Collagenase, Type 1 LS004194 100 mg
胶原酶,I型 Collagenase, Type 1 LS004196 1 gm
胶原酶,I型 Collagenase, Type 1 LS004197 5 gm
胶原酶,II型 Collagenase, Type 2 LS004174 100 mg
胶原酶,II型 Collagenase, Type 2 LS004176 1 gm
胶原酶,II型 Collagenase, Type 2 LS004177 5 gm
胶原酶,III型 Collagenase, Type 3 LS004180 100 mg
胶原酶,III型 Collagenase, Type 3 LS004182 1 gm
胶原酶,III型 Collagenase, Type 3 LS004183 5 gm
胶原酶,IV型 Collagenase, Type 4 LS004186 100 mg
胶原酶,IV型 Collagenase, Type 4 LS004188 1 gm
胶原酶,IV型 Collagenase, Type 4 LS004189 5 gm

Crude collagenase preparations contain several isoforms of two different collagenases, a sulfhydryl protease, clostripain, a trypsin-like enzyme, and an aminopeptidase. This combination of collagenolytic and proteolytic activities is effective at breaking down intercellular matrices, the essential part of tissue dissociation. One component of the complex is a hydrolytic enzyme which degrades the helical regions in native collagen preferentially at the Y-Gly bond in the sequence Pro-Y-Gly-Pro, where Y is most frequently a neutral amino acid. This cleavage yields products susceptible to further peptidase digestion. Crude collagenase is inhibited by metal chelating agents such as cysteine, EDTA or o-phenanthroline but not DFP. It is also inhibited by α2-macroglobulin, a large plasma glycoprotein. Ca2+ is required for enzyme activity. Particular enzymatic profiles of each collagenase have been correlated with the tissues from which the cells for study were obtained (or with the uses to which the cells are put) and as a result of the correlations several types of crude collagenases have been established by Worthington: Types 1, 2, 3, and 4.

 Type 1 crude collagenase has the original balance of collagenase, caseinase, clostripain and tryptic activities.
• Type 2 contains higher relative levels of protease activity, particularly clostripain.
• Type 3 contains lowest levels of secondary proteases.
• Type 4 is designed to be especially low in tryptic activity to limit damage to membrane proteins and receptors.
• Purified collagenase, Code: CLSPA, contains minimal secondary proteolytic activities along with high collagenase activity.