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Recombinant Human Interleukin-1 Receptor Antagonist
(rHuIL-1RA)
Source: Escherichia coli.
Molecular Weight: Approximately 17.1 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.
Quantity: 20µg/100µg/1000µg
AA Sequence: RPSGRKSSKM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNV NLEEKIDVVP IEPHALFLGI
HGGKMCLSCV KSGDETRLQL EAVNITDLSE NRKQDKRFAF IRSDSGPTTS FESAACPGWF
LCTAMEADQP VSLTNMPDEG VMVTKFYFQE DE
Purity: > 96 % by SDS-PAGE and HPLC analyses.
Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α-
dependent proliferation of murine D10S cells is less than 40 ng/ml, corresponding to a specific
activity of > 2.5 × 104
IU/mg in the presence of 50 pg/ml rHuIL-1α.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Endotoxin: Less than 1 EU/µg of rHuIL-1RA as determined by LAL method.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a
concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage,
preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For
maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to
-70 °C. Avoid repeated freeze/thaw cycles.
Usage: This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further
evaluation purposes. NOT FOR HUMAN USE.
Human Interleukin-1 Receptor Antagonist
Interleukin-1 receptor antagonist (IL-1RA) is a member of the IL-1 family. IL-1RA is secreted by various types of cells including
immune cells, epithelial cells, and adipocytes, and is a natural inhibitor of the pro-inflammatory effect of IL-1β. This protein
inhibits the activities of interleukin 1, alpha (IL-1α) and interleukin 1, beta (IL-1β), and modulates a variety of interleukin 1
related immune and inflammatory responses. IL-4 and IL-13 have been shown to amplify the stimulatory effect of IL1-beta on
the production of soluble and intracellular forms of IL1-RA. The regulated expression of IL-1RA in various cell types has been
shown to be influenced by cytokines. In synovial fibroblasts the synthesis of IL-1RA is markedly enhanced by IL-1, TNF-α, or
PDGF. The protein shows 26 % amino acid homology to IL-1β and 19 % homology to IL-1α.