点击图片查看原图
Recombinant Human Interleukin-1 beta
(rHuIL-1β)
Source: Escherichia coli.
Molecular Weight: Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.
Quantity: 2µg/10µg/1000µg
AA Sequence: APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIP
VAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQ
FPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS
Purity: > 97 % by SDS-PAGE and HPLC analyses.
Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation
assay using murine D10S cells is less than 1.0 pg/ml, corresponding to a specific activity of > 1.0 ×
109
IU/mg.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Endotoxin: Less than 1 EU/µg of rHuIL-1β as determined by LAL method.
Reconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a
concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage,
preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For
maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to
-70 °C. Avoid repeated freeze/thaw cycles.
Usage: This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further
evaluation purposes. NOT FOR HUMAN USE.
Human Interleukin-1 beta
Interleukin-1 beta (IL-1β) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as
neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one
of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar
but not identical biological properties; The mature human IL-1β shares 96% amino acid sequence identity with rhesus and 67 % -
78 % with canine, mouse and rat IL-1β.