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| Tb-3123 | 细菌蛋白提取试剂盒 | 50 assays | 600 |
| Tb-3123A | 细菌蛋白提取试剂盒 | 100 assays | 1,000 |
| 革兰氏阳性菌和革兰氏阴性菌通用 |
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Description: Bacteria Protein Extraction Kit is designed for the lysis of bacterial cells for the purification of recombinant and wild type proteins. It is useful for the preparation of high purity inclusion bodies. There is no need for special equipment such as sonicator. This method is fast and obtains high yields of protein. The gentle, non-ionic extraction method can be used to extract functionally soluble proteins without a need for secondary treatment such as sonication or freeze/thaw. It is compatible with the FLAG, poly-His and GST fusion protein affinity chromatography purification systems, the kit is also compatible with many enzyme or protein assays and compatible with protease inhibitor cocktails. |
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Applications: The kit provides reagent for extracting bacterial protein from 1~5 liter culture and 10 or 50 extractions. |
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Features: Simple & Fast, not need for special equipment. It is suitable for long term storage. |
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Storage Condition: Store 10 x Cell lysis buffer at 4℃; DNase I, RNase, lysozyme and PMSF should be stored at-15~-20℃. |
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一步法细菌活性蛋白提取试剂盒 One Step Bacterial Active Protein Extraction Kit |
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产品说明：一步法细菌活性蛋白提取试剂盒采用一种温和的可以通过透析去除的,破裂细菌细胞，溶解蛋白，而不会产生变性的非离子型去污试剂从大肠杆菌中提取可溶性蛋白，不需要超声等机械细胞破碎方法。该试剂可以从细菌裂解液中提取可溶性蛋白和回收包涵体蛋白，而且提取效率要高于超声等机械细胞破碎方法。该试剂盒提供的Lysozyme和DNase I可以提高分子量大于60 kDa可溶性蛋白和蛋白包涵体沉淀的洗涤回收效率，提取的可溶性蛋白可以直接用于电泳、免疫共沉淀、蛋白质交链、标记、Pull Down、EMSA、酶免分析（Western Blot、ELISA、RIA）等实验。由提取试剂、Lysozyme、Dnase、PMSF和DTT组成。储存条件：2~8℃。 产品特点： 1、可以在20 min内完成对细菌可溶性蛋白的提取； 2、该试剂可以同时实现可溶性蛋白提取和蛋白包涵体洗涤、沉淀和回收； 3、提取的可溶性蛋白可以直接用于电泳、免疫共沉淀、蛋白质铰链、标记、Pull Down、EMSA等实验； 4、提取的蛋白质可以透析去除离子和去污试剂，或将缓冲液替换为所需要的合适缓冲液； 5、可以按照需要按比例扩大提取的菌体的量，比较合适于重组蛋白的快速筛选。 |
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