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| Tb-2346 | 抗荧光淬灭封片剂 | 5 / 10 ml | 130 / 220 |
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ProLong® antifade mountants suppress photobleaching and preserve the signals of your fluorescently labeled target molecules for long-term storage and analysis. ProLong® reagents cure within 24 hours, forming a semi-rigid gel with a refractive index of 1.46. Mounted samples can be saved for months with appropriate storage. Key benefits of ProLong® antifade reagents include:
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- For immediate analysis and short-term storage see SlowFade® reagents
- For long-term analysis of Alexa Fluor® dyes see ProLong® Gold products
- For long-term analysis of all dyes and fluorescent proteins see ProLong® Diamond products
Performance of ProLong® antifade reagents
ProLong® Gold antifade reagent performance
ProLong® Gold antifade reagent has become a widely used antifade reagent for Molecular Probes® high-performance fluorescent dyes and stains because it preserves signal across the entire visible spectrum and causes little or no quenching of the initial signal. ProLong® Gold is room-temperature stable—keep it on the bench, ready to use in your next imaging experiment.
ProLong® Diamond antifade reagent performance
ProLong® Diamond antifade reagent delivers all the performance of ProLong® Gold reagent with Alexa Fluor® dyes and provides additional protection for traditional dyes and fluorescent proteins. ProLong® Diamond reagent is a superior antifade and mountant, offering protection across the visible spectrum. Keep ProLong® Diamond reagent refrigerated for the best performance.
A 60-second time-lapse showing the enhanced resistance to photobleaching afforded by ProLong® antifade reagents. Fixed HeLa cells were labeled with fluorescein phalloidin and mounted in ProLong® Diamond reagent, ProLong® Gold reagent, or 50% PBS/glycerol. Images were acquired at 12-second intervals using a 20 objective with continuous illumination from a standard 100-watt Hg-arc lamp.
Photobleaching resistance for fluorescent dyes and proteins with ProLong® antifade reagents. Photobleaching resistance was quantified on a LSM 710 (Zeiss) confocal microscope. HeLa or U2OS cells were stained and mounted using standard ICC protocols. Five regions within three fields of view were scanned 50 with a 1.58 μs dwell time per pixel. Excitation wavelength and intensity were optimized for the fluorophore being measured.
ProLong® reagent gallery
Find the right ProLong® antifade reagent
Performance across the spectrum
ProLong® Diamond reagent is a superior antifade and mountant, offering protection across the visible spectrum and exhibiting less initial quenching of the fluorescent signal. It provides additional protection for traditional dyes such as FITC and fluorescent proteins such as GFP. In a comparison of commercial hard-mount antifade reagents in a standardized experiment, ProLong® mountants outperformed other products across the analysis spectrum.
Comparison of degree of photobleaching protection by ProLong® antifades for various fluorescent dyes
| Fluorescent dye | Ex/Em (nm) | Resistance to photobleaching* | ||
|---|---|---|---|---|
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ProLong® Gold |
ProLong® Diamond |
VECTASHIELD® HardSet
Mounting Medium |
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| Hoechst® |
350/461
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|
90%
|
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| DAPI |
345/455
|
90%
|
90%
|
90%
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| BODIPY® FL |
505/513
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70%
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|
50%
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| Alexa Fluor® 488 |
495/519
|
80%
|
|
90%
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| GFP |
488/510
|
40%
|
90%
|
90%
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| Fluorescein |
494/518
|
80%
|
90%
|
90%
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| Cy®3 |
550/570
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80%
|
80%
|
80%
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| Alexa Fluor® 546 |
556/575
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|
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| Tetramethylrhodamine |
555/580
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80%
|
80%
|
80%
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| Alexa Fluor® 555 |
555/565
|
90%
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|
90%
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| Alexa Fluor® 568 |
578/603
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70%
|
90%
|
90%
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| Texas Red® |
595/615
|
90%
|
90%
|
90%
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| Alexa Fluor® 594 |
590/617
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|
90%
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| TO-PRO®-3 |
642/661
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90%
|
90%
|
90%
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| Alexa Fluor® 647 |
652/668
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|
90%
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0%
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| Cy®5 |
650/670
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|
90%
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| *The degree of photobleaching protection is compiled from a combination of bright field exposure and confocal scanning data to represent typical user experience in fluorescence imaging applications. VECTASHIELD is a registered trademark of Vector Laboratories, Inc., Burlingame, CA. Hoechst is a trademark of Hoechst, GmbH. Cy is a trademark of GE Healthcare UK, Ltd. |
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Green chemistry
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For added convenience and a reduction in the environmental impact associated with cold storage requirements, our antifade reagents are provided in dropper bottles with storage right at your bench. In addition, these reagents are formulated with reduced sodium azide to help minimize environmental and health hazard exposure risks. Learn more about these green chemistry initiatives |
