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产品说明 有关细胞增殖和细胞活性的研究需要准确定量测定细胞培养液中的活体细胞数量。因此，计算细胞活性的检测法对于优化细胞培养条件、评价细胞生长因子和营养成分、研发新型抗生素和药物、评价环境污染物的毒性作用和细胞介导的毒性以及研究程序性细胞死亡（细胞凋亡）都是必不可少的。本公司生产的CellQuanti-MTTTM 试剂盒为测定培养液中的活体细胞数量提供了一种方便、敏感、量化且可靠的检测方法。该匀相比色检测法是基于一种淡黄色底物四唑盐MTT可转化生成甲臜，甲臜是一种紫色显色剂。吡啶核苷酸辅因子NADH/NADPH参与了该细胞还原反应，并且只有活体细胞才能催化这种反应。反应产物甲臜为低水溶性的紫色晶状体，用增溶缓冲液来溶解甲臜可方便地定量测定产物的组成。在550 - 620 nm下测得的产物吸光强度直接与培养液中的活体细胞数量成正比。为了提高检测的敏感性、稳健性和自动化程度，试剂盒中的试剂配比恰当且进行了优化处理。
产品特点 安全：非放射性检测（参见3H-胸腺嘧啶核苷掺入法检测）。敏感且准确：可准确定量低至950个细胞。快速：采用96孔板进行高通量检测，每天同时处理成千上万的样品。匀相且简单：“混合-培养-测量”型检测法。无需冲洗或试剂移取步骤。稳健且适合高通量筛选：Z’系数≥0.5。采用高通量筛选液体处理系统该检测易于自动化。
适用范围 细胞增殖: 细胞因子、生长因子和和营养成分对活体细胞数量的影响。细胞毒性和细胞凋亡: 评价有毒化合物、抗体、 毒素和环境污染物等。 药物鉴定: 高通量筛选毒性药物和药物。
储存条件： 试剂在-20°C下保存。缓冲液和增溶剂分别在4°C和室温下保存。
Description The study of cell proliferation and cell viability requires the accurate quantification of the number of viable cells in a cell culture. Therefore, assays for calculating cell viability are necessary for optimizing cell culture conditions, evaluating cell growth factors and nutrients, discovering novel antibiotics and anti-cancer drugs, evaluating toxic effects of environmental pollutants and cell mediated toxicity and studying programmed cell death (apoptosis).The CellQuanti-MTTTM assay kit provides a convenient, sensitive, quantitative and reliable assay for determining the number of viable cells in a given culture. This homogeneous colorimetric assay is based on the conversion of a tetrazolium salt MTT, a pale yellow substrate, to formazan, a purple dye. This cellular reduction reaction involves the pyridine nucleotide cofactors NADH/NADPH and is only catalyzed by living cells. The formazan product has a low aqueous solubility and is present as purple crystals. Dissolving the resulting formazan with a solubilization buffer permits the convenient quantification of product formation. The intensity of the product color, measured at 550 - 620 nm, is directly proportional to the number of living cells in the culture. Reagents in the kit have been carefully formulated and optimized for sensitivity, assay robustness and automation.
Key features Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay).Sensitive and accurate. As low as 950 cells can be accurately quantified.Fast. High-throughput assay using 96-well plates allows simultaneous processing tens of thousands of samples per day.Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.Robust and amenable to HTS. Z’ factors of 0.5 and above are observed. Can be readily automated with HTS liquid handling systems.
Applications Cell Proliferation: effects of cytokines, growth factor, nutrients.Cytotoxicity and Apoptosis:evaluation of toxic compounds, anti-cancer antibodies, toxins, environmental pollutants etc. Drug Discovery: high-throughput screen for toxic and anticancer drugs.
Storage conditions. Store the Reagent at -20 °C. Keep Assay Buffer and Solubilization Solution at 4 °C and room temperature, respectively. Shelf life: 12 month.
其他 :编号：CQMT-048, CQMT-500
测试：48T, 500T