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测定方法 比色法(570nm)或荧光法(530/585nm)定量测定过氧化氢酶的含量。
产品说明 过氧化氢酶（EC 1.11.1.6）是一种普遍存在的酶，主要作用是催化过氧化氢(H2O2)分解为水和氧气。机体中许多重要的细胞活动会产生副产物H2O2，而过氧化氢酶是通过阻止H2O2的过量产生来保证这些细胞活动安全进行。灰色头发、过氧化物酶体异常及过氧化物酶缺乏等症状与过氧化氢酶活性的缺乏或不足有关。因此一种简便、直接、高通量的检测过氧化氢酶活性的方法将有广泛的应用，本公司的改良产品是使用一种氧化还原显色剂来直接测定过氧化氢酶对H2O2的降解，其在吸光度（570nm）或荧光强度（530/585nm）直接与样品中的过氧化氢酶活性成正比，可高效检测酶活。
产品特点 灵敏性和准确性：仅需10 µL 样品。线性检测范围：0.2-5 U/L 过氧化氢酶。简单方便：添加酶底物至样品中，温育30 min，接着使用检测试剂，读取光密度值或荧光密度值。
适用范围 直接检测：生物样品（如血清、血浆、尿液、细胞组织等）的过氧化氢酶活性。药物鉴定/药理学：药物对过氧化氢酶活性的作用。
贮藏条件： 试剂盒应冷冻运输，所有试剂均于-20°C保存。签收后6个月内有效。
Description CATALASE (EC 1.11.1.6), is an ubiquitous antioxidant enzyme that catalyzes the decomposition of hydrogen peroxide (H2O2) to water and oxygen.By preventing excessive H2O2 build up, catalase allows important cellular processes which produce H2O2 as a byproduct to occur safely. Deficiency in catalase activity has been associated with grey hair and peroxisomal disorder acatalasia. Simple, direct and high-throughput assays for catalase activity find wide applications. our improved assay directly measures catalase degradation of H2O2 using a redox dye. The change in color intensity at 570nm or fluorescence intensity (lem/ex = 585/530nm) is directly proportional to the catalase activity in the sample.
Key features Sensitive and accurate. Use 10 mL sample. Linear detection range 0.2 to 5 U/L catalase activity.Simple and Convenient. The procedure involves adding a Substrate to sample, incubation for 30 min, followed by a Detection Reagent and reading the optical density or fluorescence intensity.
Applications: Direct Assays: catalase activity in biological samples e.g. serum, plasma, urine, saliva, cell culture etc.Drug Discovery/Pharmacology: effects of drugs on catalase activity.
Storage conditions. The kit is shipped on ice. Store all components at -20°C. Shelf life of three months after receipt.
其他 :编号：ECAT-048,ECAT-100
测试：48T,100T