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产品说明 α-葡萄糖苷酶可水解非还原性的1,4-α-D-葡萄糖残基末端，并生成α-D-葡萄糖。动物依靠α-葡萄糖苷酶将食物中的麦芽糖水解成葡萄糖后吸收。α-葡萄糖苷酶的活性异常可能与、庞佩氏症等疾病有关。在科研和药物研究中，方便、直接、自动化的α-葡萄糖苷酶活性检测手段越来越受人们欢迎。本公司研制的α-葡萄糖苷酶检测试剂盒可直接用于检测生物样品中的α-葡萄糖苷酶活性，免除了预处理过程。该改良方法利用底物试剂被α-葡萄糖苷酶水解成黄色的产物, 其反应速度与酶的活性成正比。
产品特点 敏感度高、检测范围广：采用20μL样本，线性检测范围在2-250 U/L。操作简便：仅用单一“混合-测量”步骤，可以在20分钟内准确量化α-葡萄糖苷酶的活性。高通量：所有试剂均可用于高通量液体处理设备。
产品用途 1．直接检测生物样本中的α-葡萄糖苷酶活性。2．用于α-葡萄糖苷酶产品的鉴定与质量控制。3．用于药品研发中的高通量筛选以及对α-葡萄糖苷酶抑制剂效果的评估。
储藏条件 可在室温下运输。所有试剂需在-20℃保存。
DEscriptION α-GLUCOSIDASE hydrolyzes the terminal, non-reducing 1,4-linked α-D-glucose residues with release of α-D-glucose. α-Glucosidase is needed by all animals to hydrolyze maltose to glucose for use as a food. Aberrant activities have been implicated in diseases such as diabetes and Pompe disease. Simple, direct and automation-ready procedures for measuring α-glucosidase activity are becoming popular in Research and Drug Discovery. Our α-Glucosidase Assay Kit is designed to measure α-glucosidase activity directly in biological samples without pretreatment. The rate of the reaction is directly proportional to the enzyme activity.
KEY FEATURES High sensitivity and wide linear range. Use 20 μL sample. The detection limit is 2 U/L, linear up to 250 U/L. Homogeneous and simple procedure. Simple “mix-and-measure” procedure allows reliable quantitation of α-glucosidase activity within 20 minutes.
Robust and amenable to HTS. All reagents are compatible with highthroughput liquid handling instruments.
APPLICATIONS 1. Direct Assays: α-glucosidase activity in biological samples. 2. Characterization and Quality Control for α-glucosidase production. 3. Drug Discovery: high-throughput screen and evaluation of α-glucosidase inhibitors.
STORAGE CONDITIONS The kit is shipped at room temperature. Store all components at -20°C. Shelf life of at least 6 months.
其他 :编号：DAGD-048,DAGD-100
测试：48T,100T