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测定方法 比色法（585nm）定量测定α-淀粉酶活性。
产品说明 淀粉酶是一种水解酶，它能够作用于淀粉分子中的a-1，4-糖苷键，将其分解为葡萄糖。a-淀粉酶可以作用于直链淀粉和支链淀粉，切开淀粉链使其转化成麦芽糖、麦芽三糖、葡萄糖和极限糊精。对哺乳动物来说，a-淀粉酶是主要的消化酶，其含量增高可能与涎腺损伤、腮腺炎、胰腺炎或肾衰竭等疾病有关。在科研和药物开发中，简单、自动化的a-淀粉酶检测手段是理想的。本公司的的a-淀粉酶检测包含两个步骤：(1) a-淀粉酶将样品中的淀粉水解，其产物在a-葡糖苷酶和葡糖氧化酶的作用下，转变为葡萄糖和双氧水。(2) 利用一种比色试剂，检测出双氧水浓度。
产品特点 检测敏感、准确：用96孔板检测，a-淀粉酶活性检测范围为0.3 - 50 U/L。操作简便：整个检测过程为：加入一种工作试剂并反应15分钟，然后加入检测试剂再反应20分钟后，在585nm下读取吸光度。
产品用途 直接检测血液、唾液、尿液、谷物和其他农产品中a-淀粉酶的活性。
储藏条件： 冰运。底物和检测试剂在4°C的环境下保存，其它试剂在-20°C下保存。
Description Amylase belongs to the family of glycoside hydrolase enzymes that break down starch into glucose molecules by acting on α-1,4-glycosidic bonds. The α-amylases (EC 3.2.1.1) cleave at random locations on the starch chain, ultimately yielding maltotriose and maltose, glucose and "limit dextrin" from amylose and amylopectin. In mammals, α-amylase is a major digestive enzyme. Increased enzyme levels in humans are associated with salivary trauma, mumps due to inflammation of the salivary glands, pancreatitis and renal failure.Simple, direct and automation-ready procedures for measuring amylase activity are very desirable. our EnzyChromTM a-amylase assay method involves two steps: (1). α-amylase in the sample hydrolyzes starch and the product is rapidly converted to glucose by α-glucosidase and hydrogen peroxide by glucose oxidase; (2). hydrogen peroxidconcentration is determined with a colorimetric reagent.
Applications Direct assays of α-amylase activity in blood, saliva, urine, grains and other agricultural samples.
Key features Sensitive and accurate. Linear detection range 0.3 to 50 U/L α-amylase in 96-well plate assay.
Convenient. The procedure involves adding a single working reagent, incubation for 15 min, followed by the detection reagent and a 20-min incubation and reading the optical density at 585 nm.
Storage conditions. Kit is shipped on dry ice. Store Substrate and Detection Reagent at 4°C and others at -20°C. Shelf life of at least 6 months (see expiry dates on labels).
其他 :编号：ECAM-048,ECAM-100
测试：48T,100T