点击图片查看原图
产品说明 乙酰胆碱酯酶（EC3.1.1.7, AChE），也被称为血红细胞胆碱酯酶，主要存在于血液和神经突触中。血清胆碱酯酶活性低可能是由于接促杀虫剂，或由于基因缺陷而引起的。乙酰胆碱酯酶主要将神经递，即乙酰胆碱，水解成胆碱和乙酸，从而将神经元从活性状态恢复到惰性状态。细胞或血浆中胆碱脂酶的浓度是一个十分重要的指标，在接触磷农药的环境下，可凭此确立必要的安全指标，同时也是判断工作人员是否应该远离该区域的重要依据。简单，直接，自动化的乙酰胆碱酯酶检测手段是理想的。本公司的乙酰胆碱酯酶检测试剂盒是基于Ellman法改良而成。利用AChE催化得到的硫代胆素，与显色剂5,5-二硫代双(2-硝基苯甲酸)反应呈现出黄色，通过测定产物颜色，从而计算样本中酶的活性。
产品特点 测量敏感、准确：在96孔板中检测，乙酰胆碱酯酶的活性检测范围为10-600 U/L之间。操作简便：只需加入单一反应试剂，然后室温下分别在反应后的2分钟和10分钟读取仪器数据即可。高通量：通过96孔板上的高通量自动检测手段，每天可以测试上千个样本。
产品用途 可直接检测血液，血清，血浆和其它生物样本中乙酰胆碱酯酶的活性。评价乙酰胆碱酯酶抑制剂效果。
储藏条件 室温下保存。
DEscriptION ACETYLCHOLINESTERASE (EC 3.1.1.7, AChE), also known as RBC cholinesterase, is found primarily in the blood and neural synapses. Low serum cholinesterase activity may relate to exposure to insecticides or to one of a number of variant genotypes. AChE catalyzes the hydrolysis of the neurotransmitter acetylcholine into choline and acetic acid, a reaction necessary to allow a cholinergic neuron to return to its resting state after activation. Cholinesterase levels of cells and plasma are used as a guide in establishing safety precautions relative to exposure and contact, as well as a guide in determining the need for workers to be removed from areas of contact
with the organic phosphate insecticides. Simple, direct and automation-ready procedures for measuring AChE activity are very desirable. Our Acetylcholinesterase Assay is based on an improved Ellman method, in which thiocholine produced by the action of acetylcholinesterase forms a yellow color with 5,5’-dithiobis(2-nitrobenzoic acid). The intensity of the product color, measured at 412 nm, is proportionate to the enzyme activity in the sample.
APPLICATIONS 1. Direct assays of acetylcholinesterase activity in blood, serum, plasma, and other biological samples. 2. evaluation of acetylcholinesterase inhibitors.
KEY FEATURES Sensitive and accurate. Detection range 10-600 U/L AChE activity in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at 2 min and 10 min at room temperature. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
STORAGE CONDITIONS Store all reagents at room temperature. Shelf life of at least 6 months.
其他 :编号：DACE-048,DACE-100
测试：48T,100T