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测定方法 比色法(570nm)或荧光法(530/585nm)定量测定乙酰胆碱的含量。
产品说明 乙酰胆碱是乙酰胆碱神经元产生的一种神经递质。它在骨骼肌的运动、平滑肌和心肌的调节、以及对学习、记忆和情绪都起着重要的作用。本公司生产的产品为测定生物样品中的乙酰胆碱浓度提供了一种简便、直接、高通量的检测方法。在该项检测中，乙酰胆碱被乙酰胆碱脂酶水解成胆碱，而胆碱又被胆碱氧化酶氧化成甜菜碱和H2O2。生成的H2O2与一种特殊的显色剂反应形成一种粉红色产物，其吸光强度（570nm）或荧光强度（530/585nm）直接与样品中的乙酰胆碱浓度成正比。
产品特点 检测范围：比色法10-200 uM，荧光法0.4-10 uM乙酰胆碱。
产品用途 直接检测生物样品如血清、血浆、尿液、唾液、乳汁、组织和细胞培养物中的乙酰胆碱浓度; 药物鉴定/药理学：药物对乙酰胆碱代谢的影响。
储存条件： 冰运。所有试剂均在-20°C下保存。
DEscriptION ACETYLCHOLINE is a neurotransmitter produced in acetylcholinergic neurons. It plays important roles in skeletal muscle movement, regulation of smooth and cardiac muscles, as well as in learning, memory and mood. BioAssay Systems method provides a simple, direct and highthroughput assay for measuring acetylcholine in biological samples. In this assay, acetylcholine is hydrolyzed by acetylcholinesterase to choline which is oxidized by choline oxidase to betaine and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the acetylcholine concentration in the sample.
APPLICATIONS Assays: acetylcholine in biological samples such as serum, plasma, urine, saliva, milk, tissue, and cell culture. Drug Discovery/Pharmacology: effects of drugs on acetylcholinemetabolism.
KEY FEATURES Linear detection range: colorimetric assay 10 to 200 uM, fluorimetric assay 0.4 to 10 uM acetylcholine.
STORAGE CONDITIONS The kit is shipped on ice. Store all components at -20°C. Shelf life of three months after receipt.
其他 :编号：EACL-048, EACL-100
测试：48T, 100T