测定方法: 比色法(565nm)定量测定D-乳酸的含量。
产品说明: 乳酸是在缺氧或厌氧条件下由乳酸脱氢酶(LDH)催化生成。因此乳酸浓度的监测是组织内氧需求与利用平衡的良好指标。同时
对细胞和动物生理学研究也很重要。D-乳酸仅少量存在于动物体内并且对动物样品中D-乳酸的测量常作为评定细菌感染与否。
简单,直接和自动化的乳酸浓度检测方法是非常可取的。博世生物技术提供的D-乳酸测试盒是基于乳酸脱氢酶催化氧化乳
酸,其中形成的NADH耦合到甲臜(MTT)发色体上。产物的颜色强度(565nm)与样品中乳酸的浓度成正比。
产品特点: 敏感、准确: 96-孔板检测范围为0.05~2 mM乳酸,对于含有酚红的细胞培养物样品,检测范围为0.1 ~1 mM 乳酸。
储存条件: 所有试剂均于-20°C 保存。
DEscriptION Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for Dlactate
in animal samples is a means to determine the presence of bacterial infection.
KEY FEATURES Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM D-lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1mM D-lactate in 96-well plate assay.
Storage conditions Store all reagents at -20°C. Shelf life: 6 months after receipt.
其他 :
编号:EDLC-048, EDLC-100
测试:48T, 100T