点击图片查看原图
测定方法 比色法（565nm）定量测定乙醇的浓度。
产品说明 含酒精的饮料属于日常饮用的饮料。研究表明，酗酒可能导致各种形式的肝脏疾病和促进死亡率的上升。定量测定酒精(乙醇，C2H5OH)浓度被广泛应用在基础研究，药物开发，临床研究和酒类制造业。简单，直接，自动化测量乙醇浓度方法是非常可取的。博世生物技术公司的乙醇测试盒是基于乙醇脱氢酶催化氧化乙醇，其中形成的NADH耦合到甲臜(MTT)发色体上。产物的颜色强度(565nm)与样品中乙醇的浓度成正比。
适用范围 直接检测：生物样品如血清、血浆、尿液、唾液中的酒精。药理学: 药物对酒精代谢的影响。
产品特点 灵敏、准确: 检测下限为0.0008 vol % (140 mM or 8 ppm),线性度高达0.1%酒精(96-孔板法)。使用方便：仅添加单一工作试剂，培养30分钟，不需加热器。高通量筛选：通过96-孔板高通量的自动化检测，每天可以检测上千份样品。
贮藏条件：室温下储存终止液，其他所有试剂均于-20°C保存。
Description Alcoholic drinks are among the daily consumed beverages. Studies have shown heavy alcohol consumption may lead to various forms of liver diseases and to increased mortality rates. Quantitative determination of alcohol (ethanol, C2H5OH) has applications in basic research, drug discovery, clinic studies and in the alcoholic industry. Simple, direct and automation-ready procedures for measuring ethanol concentration are very desirable. our EnzyChromTM ethanol assay kit is based on alcohol dehydrogenase catalyzed oxidation of ethanol, in which the formed NADH is coupled to the formazan (MTT) chromogen. The intensity of the product color, measured at 565 nm, is proportionate to the ethanol concentration in the sample.
Applications Direct Assays: ethanol in serum, plasma, urine and saliva samples. Pharmacology: effects of drugs on alcohol metabolism.
Key features Sensitive and accurate. Detection limit 0.0008 vol % (140 mM or 8 ppm), linearity up to 0.1% ethanol in 96-well plate assay.Convenient. The procedure involves adding working reagent, incuba-ting for 30 min and stropping reaction. No 37°C heater is needed.High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Storage conditions. Store Stop Reagent at room temperature and all other reagents at -20°C. Shelf life of at least 6 months.
其他 :编号：ECET-048, ECET-100
测试：48T, 100T