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测定方法 比色法（570nm）或荧光法（530nm/590nm）定量测定甘油的浓度。
产品说明 甘油(又称丙三醇, C3H5(OH)3)广泛应用于食品，饮料和药物配方中。它也是生物柴油生产中主要的副产品。简单，直接，自动化的甘油浓度测定得到了广泛的应用。本公司生产的甘油试剂盒仅需添加含有甘油激酶，甘油磷酸氧化酶和显色剂的单一工作试剂。其吸光强度(570nm) 或荧光强度(lem/ex = 585/530 nm）与样品中的甘油浓度成正比。
产品特点 灵敏、准确：仅需10 μL样品，96孔板测试范围10 – 1000 μM (92 mg/dL-9.2 mg/dL)(比色法)或2 – 50 μM (荧光法)。简单、方便： 仅需添加单一工作试剂和室温培养20分钟的步骤 , 适合高通量测试。试剂稳定：优化后的测定法大大提高了试剂和信号的稳定性。
适用范围 直接检测：生物样品(如血清和血浆)中甘油的含量。药品/药理学：药物对甘油代谢的影响。食品、饮料: 食品、饮料和药物配方中的甘油。
贮存条件： 冰运，缓冲液在4°C下保存，其余试剂在 -20°C保存。
Description Glycerol [Glycerin or Glycerine, C3H5(OH)3] is widely used in foods, beverages and pharmaceutical formulations. It is also a main by-product of biodiesel production. Simple, direct and automation-ready procedures for measuring glycerol concentrations find wide applications. our glycerol assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at lem/ex = 585/530nm is directly proportional to glycerol concentration in the sample.
Key features Sensitive and accurate. Use as little as 10 mL samples. Linear detection range in 96-well plate: 10 to 1000 mM (92 mg/dL to 9.2 mg/dL) glycerol for colorimetric assays and 2 to 50 mM for fluorimetric assays.Simple and convenient. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature, compatible for HTS assays.Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.
Applications: Direct Assays: glycerol in biological samples (e.g. serum and plasma).Drug Discovery/Pharmacology: effects of drugs on glycerol metabolism. Food and Beverages: glycerol in food, beverages, pharmaceutical formulations etc.
Storage conditions. The kit is shipped on dry ice. Store Assay Buffer at 4°C and other reagents at -20°C. Shelf life of three months after receipt
其他 :编号：EGLY-048，EGLY-200
测试：,48T,200T