点击图片查看原图
测定方法 比色法（570nm）或荧光法（585nm/530nm）定量测定氨/铵离子的浓度。
产品说明：丙酮酸在细胞代谢中起关键作用。丙酮酸可以通过把葡萄糖转化为碳水化合物，通过乙酰辅酶A转化为脂肪或能量，也可转化为丙氨酸和乙醇。人体内丙酮酸的非正常水平与肝脏疾病和代谢疾病有关。简单、直接、自动化测量丙酮酸浓度的手段，在研究和药物研发中受到广泛的应用。我公司生产的丙酮酸测试方法使用单一工作试剂将丙酮酸氧化和过氧化氢的检测一次性完成。在波长为570nm检测的颜色强度或 lem/ex = 585/530nm处的荧光强度与样品中丙酮酸浓度成正比。
产品特点 灵敏、准确：只需10 mL样本。96孔板中检测范围：比色法2 - 500 mM， 荧光法0.2 - 50 mM丙酮酸。简单、方便： 该过程包含了添加单一工作试剂，在温室下培养30分钟。改良了试剂的稳定性：优化的配方，大大增加了试剂和信号稳定。
产品用途: 可直接检测生物样品中的丙酮酸。用于药品和药理学研究，检测药物对丙酮酸代谢的影响。
储存条件： 冰运，在 -20°C下储藏。
Description Pyruvate is a key intermediate in cellular metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders. Simple, direct and automation-ready procedures for measuring pyruvate concentrations find wide applications in research and drug discovery. our pyruvate assay uses a single Working Reagent that combines pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at lem/ex = 585/530nm is directly proportional to pyruvate concentration in the sample.
Key features Sensitive and accurate. Use as little as 10 mL samples. Linear detection range in 96-well plate: 2 to 500 mM (17 mg/dL to 4.4 mg/dL) pyruvate for colorimetric assays and 0.2 to 50 mM for fluorimetric assays.Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature, compatible for HTS assays.Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.
Applications: Direct Assays: pyruvate in biological samples.Drug Discovery/Pharmacology: effects of drugs on pyruvate metabolism.
Storage conditions. The kit is shipped on dry ice. Store all reagents at -20°C. Shelf life of three months after receipt.
其他 :编号：EPYR-048，EPYR-100
测试：48T,100T