测定方法 比色法(570nm)定量测定甘油三酯的含量。
产品说明 甘油三酸脂是植物油和动物脂肪的主要成分,是一种重要的能量来源和食物脂肪载体。在人体中,甘油三酸脂含量过高可能与动脉粥样硬化、心脏病或胰腺炎等疾病有关。在科研和药物研究中,方便、直接、自动化的甘油三酸脂含量检测手段获得了广泛的应用。本公司研制的甘油三酸脂检测试剂盒采用一种工作试剂,将甘油三酸脂的分解与甘油含量的测定合并到同一步骤。在此过程中染色剂氧化生成有色产物,在波长为570nm下检测,其颜色深浅程度与样品中甘油三酸脂的含量成正比。
产品特点 测量敏感、准确:用10μL样品。甘油三酸脂的线性检测范围为0.01—1.0mmol/L(0.88 mg/dL—88.5mg/dL)。简单、快捷:整个测试过程只需加入一种工作试剂,并在室温下培养30分钟,也可用于高通量检测。试剂稳定性高:产品经过配方的改良,极大的提高了试剂和测试信号的稳定性。
产品用途 1. 直接检测血清、血浆等生物样品中甘油三酸脂的含量。2. 用于药品和药理学研究,检测药物对甘油三酸脂代谢的影响。
储藏条件 试剂盒冰运,缓冲液在4℃下保存,其余试剂在-20℃下保存。产品需在收到后三个月内使用。
DEscriptION TRIGLYCERIDE, also known as TRIACYLTRIGLYCERIDE or TRIACYLGLYCERIDE, is the main constituent in vegetable oil and animal fats. Triglycerides play an important role as energy sources and transporters of dietary fat. In the human body, high levels of triglycerides in the bloodstream have been linked to atherosclerosis, heart disease and pancreatitis. Simple, direct and automation-ready procedures for measuring triglyceride concentrations find wide applications in research and drug discovery. BioAssay Systems triglyceride assay uses a single Working Reagent that combines triglyceride hydrolysis and glycerol determination in one step, in which a dye reagent is oxidized to form a colored product. The color intensity at 570nm is directly proportional to triglyceride concentration in the sample.
KEY FEATURES Sensitive and accurate. Use as little as 10 μL samples. Linear detection range 0.01 mmol/L to 1.0 mmol/L (0.88 mg/dL to 88.5 mg/dL) triglyceride. Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature, compatible for HTS assays. Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.
APPLICATIONS Direct Assays: triglyceride in biological samples (e.g. serum and plasma). Drug Discovery/Pharmacology: effects of drugs on triglyceride metabolism.
STORAGE CONDITIONS The kit is shipped on dry ice. Store Assay Buffer at 4°C and other reagents at -20°C. Shelf life of three months after receipt.
其他 :编号:ETGA-048,ETGA-200
测试:48T,200T