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测定方法 比色法（溴甲酚绿BCG，620nm)定量测定白蛋白的浓度。
产品说明 白蛋白是人体血浆中含量最多的蛋白质，约占血清蛋白总量的60%。白蛋白具有重要的生理功能，包括维持胶体渗透压，并可与长链脂肪酸、胆汁酸、胆红素、血红素、钙和镁离子等物质结合。它拥有性和抗凝血性，能充当营养物质和药物的运输载体，同时也是血浆PH值的缓冲剂。血清白蛋白含量直接关系到肝脏疾病、肾脏疾病、营养不良或蛋白流失性肠道病症的发生发展与预后。血清白蛋白量过高亦可能由脱水而引起。在科研和新药开发中，方便、直接、自动化的白蛋白含量检测手段是理想的。本公司研制的BCG法血清白蛋白检测试剂盒可直接对样本检测，免除了预处理过程。测试方法是利用溴甲酚绿能与白蛋白反应产生绿色复合物的特性，在波长为630nm下检测，其颜色深浅程度与样本中白蛋白的含量对应成比例。并且通过配方的优化，充分减小了未处理的样本中其他杂质的干扰。
产品特点 敏感、准确：只用5μL样本，在96孔板中测试，测试范围在0.01-5g/dL (1.5-750μM)。 简单、高通量：整个测试过程只需加入工作试剂并反应5分钟。通过96孔板上的高通量检测手段，每天可以测试上千份样本。试剂稳定性高和多功能性：产品经过配方的改良，极大地提高了试剂和测试信号的稳定性，测试可采用比色皿或96孔板进行。
产品用途 1．直接检测血清、血浆、尿液和生物制剂中白蛋白的含量。 2．用于药品和药理学研究，检测药物对白蛋白代谢的影响。
储藏条件 可在室温下运输。试剂和标准品分别在4°C和-20°C下保存.
DEscriptION Albumin is the most abundant plasma protein in human. It accounts for about 60% of the total serum protein. Albumin plays important physiological roles, including maintenance of colloid osmotic pressure, binding of key substances such as long-chain fatty acids, bile acids, bilirubin, haematin, calcium and magnesium. It has anti-oxidant and anticoagulant effects, and also acts as a carrier for nutritional factors and drugs, as an effective plasma pH buffer. Serum albumin is a reliable prognostic indicator for morbidity and mortality, liver disease, nephritic syndrome, malnutrition and protein-losing enteropathies. High levels are associated with dehydration. Simple, direct and automation-ready procedures for measuring albumin
concentration in biological samples are becoming popular in Research and Drug Discovery. Our albumin assay kit is designed to measure albumin directly in biological samples without any pretreatment. The improved method utilizes bromcresol green that forms a colored complex specifically with albumin. The intensity of the color, measured at 620nm, is directly proportional to the albumin concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.
KEY FEATURES Sensitive and accurate. Use as little as 5 μL samples. Detection range 0.01-750 g/dL (1.5-750μM) albumin in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has
greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.
APPLICATIONS Direct assays: albumin in serum, plasma, urine, biological preparations. Drug discovery/Pharmacology: effects of drugs on albumin metabolism.
STORAGE CONDITIONS The kit is shipped at room temperature. Shelf life of at least 6 months.
其他 :编号：DIAG-048，DIAG-250
测试：48T,250T