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测定方法 比色法（612nm）测定钙离子Ca²的浓度。
产品说明 钙的含量可以反映出骨骼或钙调节紊乱的疾病。甲状旁腺功能亢进症，骨转移瘤和维他命过多症导致血清中钙离子的水平提高，而甲状旁腺功能低下，肾病，佝偻病，脂肪泻，肾炎，钙流失综合征则反映在钙离子的水平降低。尿液中钙的水平帮助临床医生了解肾脏如何处理某些甲状旁腺引起的疾病。尿液中钙的水平在肾结石的医学鉴定也是至关重要的。在科研和药物研发中，简单、直接、自动化的铜离子浓度检测手段越来越受欢迎。本公司研制的钙离子检测试剂盒不需任何预处理，可直接用于钙离子检测。试剂中phenolsulphone-phthalein 染色剂专门与游离的钙离子形成稳定的蓝色。在612nm下测得的吸光度与样本中钙的浓度成正比。优化的配方大幅减少了原样品中物质（如镁，脂肪，蛋白质和胆红素）的干扰。
产品特点 灵敏、准确：仅用 5 mL样品，线性测试范围（96孔板）： 0.08 - 20 mg/dL (0.02 - 5 mM)。简单、高通量：该过程包含了添加单一工作试剂及3分钟培养的步骤。通过高通量自动检测，每天可以测试上千个样本。试剂稳定性和多功能性提高：优化的配方，大大增加了试剂和信号的稳定性。低干扰：无需预处理，可直接用于未加工的生物样品。如存在于脂质，蛋白质和矿物质的镁，铁和锌对它干扰小。
产品用途 直接检测 血，尿，唾液等中的Ca2+。用于药品和药理学研究，检测药物对钙代谢的影响。检测食品、饮料等的钙的含量。检测水和土壤中的钙含量。
储藏条件：在室温下运输，试剂和标准品均在4°C下保存。
Description Calcium is measured to monitor diseases of the bone or calcium regulation disorders. Increased calcium levels in serum are reported in hyperparathyroidism, metastatic bone lesions and hypervitaminosis, while decreased levels are observed in hypoparathyroidism, nephrosis, rickets, steatorrhea, nephritis and calcium-losing syndromes. Urinary calcium levels aid the clinician in understanding how the kidneys handle calcium in certain diseases of the parathyroid gland. Urinary calcium levels are also essential in the medical evaluation of kidney stones.Simple, direct and automation-ready procedures for measuring calcium concentration in biological samples are becoming popular in Research and Drug Discovery. our calcium assay kit is designed to measure calcium directly in biological samples without any pretreatment. A phenolsulphonephthalein dye in the kit forms a very stable blue colored complex specifically with free calcium. The intensity of the color, measured at 612 nm, is directly proportional to the calcium concentration in the sample. The optimized formulation minimizes any interference by substances such as magnesium, lipid, protein and bilirubin.
Key features Sensitive and accurate. Use as little as 5 mL samples. Linear detection range 0.08 mg/dL (20mM) to 20 mg/dL (5mM) Ca2+ in 96-well plate assay.Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 3 min. Can be readily automated as a high-throughput assay for thousands of samples per day.Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals such as magnesium, iron and zinc.
Applications Direct Assays: Ca2+ in serum, urine, saliva etc.Drug Discovery/Pharmacology: effects of drugs on calcium metabolism. Food and Beverages: calcium determination.Environment: calcium determination in water and soil.
Storage conditions. The kit is shipped at room temperature. Store Reagent and Standard at 4°C. Shelf life of at least 6 months (see expiry dates on labels).
其他 :编号：DICA-048，DICA-500
测试：48T,500T