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测定方法 比色法（610nm）定量测定氯离子Cl- 的浓度。
产品说明 氯离子是人体中主要的细胞外阴离子，在维持机体水分平衡、渗透压及电解质平衡中发挥着重要的作用。长期呕吐、大面积烧伤、代谢性酸中毒、艾迪森氏危症或肾病变，会引起人体氯离子浓度降低。而体内氯离子浓度过高则可由脱水、充血性心力衰竭、哮喘或尿路堵塞引起。检测汗液中氯离子的含量还可以帮助我们诊断囊肿纤维变性。在科研和药物研究中，方便、直接、自动化的氯离子浓度检测手段越来越受人们欢迎。本公司研制的氯离子检测试剂盒可直接检测生物样本，免除了预处理过程。测试方法是利用试剂与氯离子反应产生有色复合物，在波长为610nm下检测，其颜色深浅程度与样本中氯离子的含量成正比。本法通过配方改良，充分减小了样品中其他杂质的干扰。
产品特点 测量敏感、准确：只需5μL样本，在 96孔板中线性测试范围在0.7-35 mg/dL (0.2-10mM)。 简单、高通量：整个测试过程只需加入一种工作试剂，利用高通量检测手段，每天可以测试上千个样本。
产品用途 1. 直接检测血清、血浆、尿液、唾液和汗液中的氯离子含量。2. 用于药理学研究，检测药物对氯离子代谢影响。3. 食品和饮料中氯离子含量的测定。4. 环境水、土壤中的氯离子含量的测定。
储藏条件 可在室温下运输。试剂在4℃环境下保存.
DEscriptION Chloride is the major extracellular anion in human body fluids. Chloride plays a key role in maintaining proper water distribution, osmotic pressure and electrolyte balance. Low chloride concentrations may be found with prolonged vomiting, extensive burns, metabolic acidosis, Addisonia crisis and renal diseases. Elevated chloride concentrations are associated with dehydration, congestive heart failure, hyperventilation and urinary obstructions. Determination of chloride in sweat is useful in diagnosing cystic fibrosis. Simple, direct and automation-ready procedures for measuring chloride concentration in biological samples are becoming popular in Research and Drug Discovery. Our chloride assay kit is designed to measure chloride directly in biological samples without any pretreatment. The improved method utilizes a reagent which forms a colored complex specifically with chloride. The intensity of the color, measured at 610nm, is directly proportional to the chloride concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.
KEY FEATURES Sensitive and accurate. Use as little as 5 μL samples. Linear detection range 0.7-35mg/dL (0.2-10mM) Cl- in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals such as magnesium, iron and zinc.
APPLICATIONS 1. Direct Assays: Cl- in serum, plasma, urine, saliva, sweat etc. 2. Drug Discovery/Pharmacology: effects of drugs on chloride metabolism. 3. Food and Beverages: chloride determination. 4. Environment: chloride determination in water and soil.
STORAGE CONDITIONS The kit is shipped at room temperature. Store Reagent and Standard at 4°C. Shelf life of at least 6 months.
其他 :编号：DICL-048,DICL-250
测试：48T,250T