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测定方法 比色法（510nm）定量测定肌酐的浓度。
产品说明 肌酐由体内肌氨酸以相对稳定比率合成，肌氨酸则来自肌肉收缩过程中产生的磷酸肌酸。血液中的肌酐通过肾脏肾小球过滤，分泌到尿液中。在健康机体中，肌酐的分泌相对稳定且不依赖饮食。对肌酐清除的检测是衡量肾小球滤过率的灵敏检验。有肾脏疾病时，血液中的肌酐水平升高，而肌酐清除率亦即尿液中肌酐减少。肌酐检测被广泛用于评估肾功能。在科研和药物研发中，简单、直接、自动化的肌酐检测手段得到了广泛应用。本公司研制的肌酐检测试剂盒不需对样品作任何预处理，可直接检测。改进的苦味酸法利用苦味酸盐与肌酐形成红色化合物。在510nm波长下测得的吸光度与样品中肌酐浓度成正比。优化的配方大幅减少了原样品中物质的干扰。
产品特点 灵敏、准确: 仅用30mL样品， 96孔板检测底限0.10 mg/dL (8mM)。简单、高通量: 仅添加单一工作试剂和培养5分钟的步骤，每天可以高通量自动检测上千份样品。试剂的稳定性和多用性更佳: 优化后的测定法大大提高了试剂和信号的稳定性。可采用比色皿或 96孔板检测。生物样品的干扰少：无需任何预处理。可直接对原始生物样品进行检测。
产品用途 直接检测: 尿液、血清、血浆和其它生物样品中肌酐的含量。药物鉴定/药理学: 药物对肌酐代谢的影响。
贮藏条件: 室温下运输，所有试剂均在2-8°C下保存，在–20°C下可长时间保存。
Description Creatinine is synthesized in the body at a fairly constant rate from creatine, which is produced during muscle contractions from creatine phosphate. In the blood, creatinine is removed by filtration through the glomeruli of the kidney and is secreted into urine. In healthy individuals, creatinine secretion is independent of diet and is fairly constant. The creatinine clearance test has become one of the most sensitive tests for measuring glomerular filtration rate. In kidney disease, creatinine levels in the blood are elevated, wheras the creatinine clearance rate and hence the urine levels are diminished. Creatinine test is most widely used to assess kidney function.Simple, direct and automation-ready procedures for measuring creatinine concentration in biological samples are becoming popular in Research and Drug Discovery. BioAssay Systems creatinine assay kit is designed to measure creatinine directly in biological samples without any pretreatment. The improved Jaffe method utilizes picrate that forms a red colored complex with creatinine. The intensity of the color, measured at 510nm, is directly proportional to creatinine concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw sample.
Key features Sensitive and accurate. Use 30 mL samples. Detection limit 0.10 mg/dL (8mM) creatinine in 96-well plate assay.Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be automated as a high-throughput assay for thousands of samples per day.Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in 96-well plate or cuvet.Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples.
Applications Direct Assays: urine, serum, plasma and biological preparations.Drug Discovery/Pharmacology: effects of drugs on creatinine metabolism.
Storage conditions. The kit is shipped at room temperature. Store all components at 2-8°C. For long-term storage, keep standard at –20°C. Shelf life: at least 6 months (see expiry dates on labels).
其他 :编号：DICT-048,DICT-500
测试：48T,500T