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测定方法 比色法（400nm）定量测定血红蛋白的含量。
产品说明 血红蛋白由四个球蛋白链组成，每一个都携带一个亚铁血红素基团。红细胞携带血红蛋白，并将氧气从肺运送到外围的组织中，以维持细胞的生存。血红蛋白的含量可以作为诊断许多疾病的依据，如、红血球增多和脱水。在科研和药物研发中，方便、直接、自动化的血红蛋白浓度检测手段是理想的。本公司研制的血红蛋白检测试方法基于改良的检测法，将血红素转化成有色产物。在波长为400 nm下检测，其颜色深浅程度与样本中血红蛋白含量对应成比例。并且通过配方的改良，充分减小了未处理的样品中其他杂质的干扰。
产品特点 敏感、准确：在96孔板中检测，血红蛋白的检测范围在0.9-200 mg/dL。简单、高通量：整个测试过程只需加入单一工作试剂，并读取吸光度。利用96孔板的高通量检测手段，每天可以测试上千份样本。安全性：所有试剂均无毒。 多功能性：检测可采用96孔板或比色皿。
产品用途 1．直接检测全血液，血清、血浆的血红蛋白含量。 2. 用于药理学研究，检测药物对血红蛋白代谢的影响。3．适用于新药开发，高通量筛选影响血红蛋白含量的药物。
储藏条件 可在室温下运输。试剂和校准品在4℃环境下保存.
DEscriptION Hemoglobin (Hb) is made of four globin chains each carrying a heme group. It is carried by red blood cells and transports oxygen from the lungs to the peripheral tissues to maintain the viability of cells. Quantitation of blood hemoglobin has been a key diagnostic parameter for various diseases such as anemia, polycythemia and dehydration. Simple, direct and automation-ready procedures for measuring hemoglobin concentration are becoming popular in Research and Drug Discovery. Our hemoglobin assay kit is based on an improved method, in which the hemoglobin is converted into a uniform colored end product. The intensity of color, measured at 400 nm, is directly proportional to hemoglobin concentration in the sample. The optimized formulation exhibits high sensitivity and substantially reduces interference by substances in the raw samples.
APPLICATIONS Direct Assays: total hemoglobin in blood, serum, plasma, urine, etc. Pharmacology: effects of drugs on hemoglobin metabolism.
Drug Discovery: HTS for drugs that modulate hemoglobin levels.
KEY FEATURES Sensitive and accurate. Linear detection range 0.9 – 200 mg /dL hemoglobin in 96-well plate assay. Simple and high-throughput. The “mix-and-read” procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Safety. Reagents are non-toxic. Versatility. Assays can be executed in 96-well plate or cuvet.
STORAGE CONDITIONS The kit is shipped at room temperature. Store reagent and calibrator at 4°C. Shelf life: at least 6 months.
其他 :编号：DIHB-048,DIHB-250
测试：48T,250T