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测定方法 比色法（620nm）定量测定磷酸根的浓度。
产品说明 磷酸盐（Pi）是自然界重要的离子之一。磷酸盐存在于所有生物系统中。它是矿物质和化肥的主要成分，也是工业废水的组成部分。因此，磷酸盐浓度的准确检测方法在药理学、生物医学研究、临床化学、工业生产监测和环境监测等领域应用广泛。 采用简单、直接、自动化的检测手段对生物和环境样品中的磷酸盐浓度进行测定被广泛应用。本公司生产的磷酸盐测试盒可直接测定样品中的磷酸盐离子(无需预处理)。这种改良后的孔雀绿方法采用了孔雀绿显色剂和钼酸盐，钼酸盐可与无机磷酸盐特异结合形成一种稳定的有色化合物。在620nm处测得的吸光强度直接与样品中的磷酸盐浓度成正比。优化后的测定法大大减少了原始样品中的物质对检测结果的干扰。
产品特点 敏感且准确：采用96孔板检测，检测范围0.30 - 50 mM（0.0028 - 0.47 mg/dL）磷酸盐。简单且高通量：检测过程中只需加入一种工作液并培养30分钟。该自动化高通量检测方法，每天可处理上千份样品。试剂的稳定性和多用性更佳：优化后的测定法大大提高了试剂和信号的稳定性。可采用比色皿或 96孔板检测。生物样品的干扰少：无需任何预处理。可直接对原始生物样品（即含有脂类、蛋白质和矿物质的样品）进行检测。
适用范围 直接检测血清、尿液、唾液、汗液、组织培养物中的Pi。药物鉴定/药理学：评价药物对Pi代谢的影响。食品及饮料: Pi含量测定。环境监测：水、土壤和化肥中的Pi测定。
储存条件：试剂盒在室温下运输。所有试剂均在4 °C下保存。
Description Phosphate (Pi) is one of the most important ion species in nature. Phosphate is present in all biological systems. It is a major constituent in minerals and fertilizers, and is a component of industrial wastewater. Thus accurate determination of phosphate concentration finds numerous applications in pharmacology, biomedical research, clinical chemistry, industrial process monitoring and environmental monitoring. Simple, direct and automation-ready procedures for measuring phosphate concentration in biological and environmental samples are becoming popular. our phosphate assay kit is designed to measure phosphate ion directly in samples without any pretreatment. The improved Malachite Green method utilizes the malachite green dye and molybdate, which forms a stable colored complex specifically with inorganic phosphate. The intensity of the color, measured at 620nm, is directly proportional to the phosphate concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.
Key features Sensitive and accurate. Linear detection range 0.30 mM (0.0028 mg/dL) to 50 mM (0.47 mg/dL) phosphate in 96-well plate assay.Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay for thousands of samples per day.Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in cuvet or 96-well plate.Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals.
Applications Direct Assays: Pi in serum, urine, saliva, sweat, tissue culture etc.Drug Discovery/Pharmacology: effects of drugs on Pi metabolism. Food and Beverages: Pi determination.Environment: Pi determination in water, soil and fertilizer.
Storage conditions. The kit is shipped at room temperature. Store all components at 4°C. Shelf life of at least 6 months (see expiry dates on labels).
其他 :编号：DIPI-048，DIPI-500
测试：48T,500T