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测定方法 比色法（425nm）定量测定锌离子Zn²的浓度。
产品说明 锌是一种重要的微量元素，在代谢中发挥着重要的作用，多种酶的活动、多种蛋白质的构成和遗传表达都与锌有关。由于锌是合成和修复DNA，RNA及蛋白质的必需物质，其含量直接影响细胞的分裂、生长、分化、发展及老化过程。缺锌常由于较低的膳食摄入量和利用率。缺锌的临床症状包括肢皮炎，免疫力低，，愈后不良，发育不全，性腺机能减退，胎儿发育不良、畸形和流产等。缺锌现已被认为与许多疾病有关，如吸收不良综合征，慢性肝病，慢性肾脏疾病，镰状细胞疾病，，和其他慢性疾病。在科研和药物研究中，方便、直接、自动化的锌含量检测手段是十分理想的。本公司研制的锌检测试剂盒可直接用于生物样品，免除了预处理过程，检测利用色原体与锌形成有色复合物特性，在波长为425nm下检测，其颜色深浅程度与样本中锌的含量成正比。
产品特点 敏感、准确：用50μL样品。在９６孔板中检测，锌的线性检测范围为0.12-10μM (0.78-65 μg/dL)。简单、高通量：通过96孔板上的高通量自动检测手段，每天可以测试上千个样本。试剂稳定性高和多功能性：产品经过配方的改良，极大的提高了试剂和测试信号的稳定性，测试可采用比色皿或96孔板。
产品用途 1. 直接检测血清、血浆（不含EDTA）、尿液或唾液中锌的含量。2. 用于药品和药理学研究，检测药物对锌代谢的影响。3. 可用于检测污水和土壤中锌的含量。
储藏条件 试剂盒可在室温下运输，试剂B和C在-20℃下保存，其余的试剂在4℃下保存。
DEscriptION Zinc is an essential trace element and plays many key roles in metabolism. It is required for the activity of more than 300 enzymes, the structure of many proteins, and control of genetic expression. Zinc status affects basic processes of cell division, growth, differentiation, development, performance and aging through its requirement for synthesis and repair of DNA, RNA and protein. The common causes of zinc deficiency are low dietary intakes and low bioavailability. Clinical signs of zinc deficiency include acrodermatitis, low immunity, diarrhea, poor healing, stunting, hypogonadism, fetal growth failure, teratology and abortion. Zinc deficiency1 has now been recognized to be associated with many diseases such as malabsorption syndrome, chronic liver disease, chronic renal disease, sickle cell disease, diabetes, malignancy, and other chronic illnesses. Simple, direct and automation-ready procedures for measuring zinc concentration in biological samples are highly desirable in Research and Drug Discovery. BioAssay Systems zinc assay kit is designed to measure zinc directly in biological samples without any pretreatment. Our method utilizes a chromogen that forms a colored complex specifically with zinc. The intensity of the color, measured at 425 nm, is directly proportional to the zinc concentration in the sample.
KEY FEATURES Sensitive and accurate. Uses 50 μL samples. Linear detection range 0.12- 10 μM (0.78-65 μg/dL) zinc in 96-well assay format. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay formats possible. Low interference in biological samples. No pretreatments are needed.
Assays can be directly performed on raw biological samples, i.e. in the presence of lipid and protein.
APPLICATIONS Direct Assays: zinc in serum, plasma (no EDTA), urine, saliva etc. Drug Discovery/Pharmacology: effects of drugs on zinc metabolism. Environment: zinc determination in waste water, soil etc.
STORAGE CONDITIONS The kit is shipped at room temperature. Shelf life: atleast 6 months.
其他 :编号：DIZN-048，DIZN-250
测试：48T,250T