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测定方法 比色法（540nm）定量测定一氧化氮的浓度。
产品说明 一氧化氮（NO）是一种活性基团，在许多关键的生理功能中发挥着重要作用。NO是一氧化氮合成酶作用于精氨酸的一种氧化产物，参与了机体防御反应和生长发育、蛋白调节及功能性生物分子间的相互作用,简单、直接、自动化的NO浓度检测手段在科研及药物研发中受到了广泛应用。NO可被氧化生成亚硝酸盐和硝酸盐，因此通常测量NO2-/NO3-的总浓度作为NO的水平。本公司生产的一氧化氮测试盒采用的是改良后的格里斯法，可准确测定硝酸盐还原成亚硝酸盐后的NO产物。该检测步骤操作简单，样品预处理和检测总共只需40分钟。
产品特点 敏感、准确：采用96孔板检测，检测范围0.1mM- 50 mM。快速、可靠：采用优化后的镉/铜（Cd/Cu）试剂，将NO3-还原成NO2-只需15分钟，且转换率>98.5%。简单、高通量：检测过程中只需将样品与2种试剂混合，培养5分钟并读取吸光度。该检测方法易于自动化，每天可处理数千份样品。
适用范围： 直接检测:血浆、血清、尿液、组织/细胞及食品中的NO浓度。药物研发/药理学：药物对NO代谢的影响。
储存条件：试剂盒在室温下运输。试剂A、试剂B和亚硝酸盐标准液在4°C下保存，其它试剂可在室温下保存。
Description Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO, an oxidation product of arginine by nitric oxide synthase, is involved in host defense and development, activation of regulatory proteins and direct covalent interaction with functional biomolecules. Simple, direct and automation-ready procedures for measuring NO are becoming popular in Research and Drug Discovery. Since NO is oxidized to nitrite and nitrate, it is common practice to quantitate total NO2-/NO3- as a measure for NO level.our QuantiChromTM Nitric Oxide Assay Kit is designed to accurately measure NO production following reduction of nitrate to nitrite using improved Griess method. The procedure is simple and the time required for sample pretreatment and assay is reduced to only 40 min.
Key features Sensitive and accurate. Detection range 0.1 - 50 mM in 96-well plate.Rapid and reliable. Using optimized Cd/Cu reagent, the time required for reduction of NO3- to NO2- is 15 min at >98.5% conversion rate. Simple and high-throughput. The procedure involves mixing sample with two reagents, incubation for 5 min and reading the optical density. Can be readily automated to measure thousands of samples per day.
Applications: Direct Assays: NO in plasma, serum, urine, tissue/cells and foods.Drug Discovery/Pharmacology: effects of drugs on NO metabolism.
Storage conditions. The kit is shipped at room temperature. Store Reagent A, B and Nitrite standard at 4°C. All other components can be stored at room temp. Shelf life: at least 6 months (see expiry dates on labels).
其他 :编号：D2NO-048，D2NO-250
测试：48T,250T