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测定方法 比色法（570nm）或荧光法（λem/ex= 585/530nm）。
产品说明 是一种类脂，也称胆甾醇，主要存在于细胞膜中，并经血液来运输。用于生成细胞膜与激素，在细胞信号传导过程中也起着重要的作用。过高的含量（高胆醇血症）与(如动脉粥样硬化等）有关；而过低的含量（低胆醇血症）则可能与抑郁症，与脑出血有关。简单，直接，自动化的含量检测手段是理想的。本公司所生产的检测试剂盒仅使用单一工作试剂，将胆甾醇酯水解、氧化及显色反应结合为一个步骤。反应产物的吸光强度（570nm）或荧光强度（λem/ex= 585/530nm）与样本中的总浓度成正比。
产品特点 检测敏感、准确： 96孔板检测的线性范围 比色法:1-100 mg/dL；荧光法: 0.2-10 mg/dL。操作简便： 室温下进行检测，无需37℃加热器。高通量： 通过96孔板上的高通量检测手段，每天可以测试上千份样本。
产品用途 可直接检测血清、血浆与其它生物样本中的含量，也可用于检测药物对代谢的影响。
储藏条件 试剂在-20℃条件下保存。
DEscriptION CHOLESTEROL is a sterol and lipid present in the cell membranes, and is transported in the bloodstream of all animals. It is used to form cell membranes and hormones, and plays important roles in cell signaling processes. Elevated levels (hypercholesterolemia) have been associated with cardiovascular diseases such as atherosclerosis; wheras, low levels (hypocholesterolemia) may be linked to depression, cancer and cerebral hemorrhage. Simple, direct and automation-ready procedures for measuring cholesterol are very desirable. Our Cholesterol Assay uses a single Working Reagent that combines cholesterol ester hydrolysis, oxidation and color reaction in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λem/ex = 585/530nm is directly proportional to total cholesterol concentration in the sample.
APPLICATIONS Direct Assays: cholesterol in serum, plasma, and other biological samples. Pharmacology: effects of drugs on cholesterol metabolism.
KEY FEATURES Sensitive and accurate. Linear detection range in 96-well plate: 1-100 mg/dL cholesterol for colorimetric assays and 0.2-10 mg/dL for fluorimetric assays. Convenient. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
STORAGE CONDITIONS Store reagents at -20°C. Shelf life of at least 6 months.
其他 :编号：E2CH-048, E2CH-100
测试：48T, 100T