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测定方法 比色法（570nm）或荧光法（530nm/590nm）定量测定高低密度(HDL/LDL/VLDL)的含量。
产品说明 高密度脂蛋白（HDL）和低密度（LDL）/极低密度（VLDL）脂蛋白中的浓度是冠心病强有力的预测证明，功能性HDL为清除细胞和动脉中 的提供保护。由于动脉粥样硬化的风险性高，低密度脂蛋白中的高浓度和高密度脂蛋白中的低浓度与有着紧密的联系。高或者低密度 脂蛋白之间的平衡完全由遗传因素决定。但是能通过药物治疗，食物选择和其他因素而得到改变。对于测量HDL和LDL/VLDL中浓度。简单﹑直接和自动化检测手段是十分理想的，博世生物技术的HDL和LDL/VLDL 定量测试盒是基于经过改良的HDL和LDL/VLDL测定方法。在570nm 处反应产物的吸光强度或者在λem/ex = 585/530nm处的荧光强度直接与样品中浓度成正比。
产品特点 灵敏准确：线性检测范围在96孔板，比色法1-100毫克/分升和荧光法0.2-10毫克/分升。方便：室温检测，无需37摄氏度加热器。
产品用途 直接测试：在血清中的HDL和LDL/VLDL。 药理研究：药物对代谢的影响。
贮存条件 室温贮存PBS和沉淀试剂，其余试剂在-20摄氏度温度下贮存。
DEscriptION CHOLESTEROL concentrations in High-Density Lipoprotein (HDL) and Low-Density (LDL)/Very-Low-Density (VLDL) Lipoproteins are strong predictors for coronary heart disease. Functional HDL offers protection by removing cholesterol from cells and atheroma. Higher concentrations of LDL and lower concentrations of functional HDL are strongly associated with cardiovascular disease due to higher risk of atherosclerosis. The balances between high- and low-density lipoproteins are solely genetically determined, but can be changed by medications, food choices and other factors. Simple, direct and automation-ready procedures for measuring HDL and LDL/VLDL concentrations are very desirable. Our HDL and LDL/VLDL quantification kit is based on our improved PEG precipitation method in which HDL and LDL/VLDL are separated, and cholesterol concentrations are determined using a single Working Reagent that combines cholesterol ester hydrolysis, oxidation and color reaction in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λem/ex = 585/530nm is directly proportional to total cholesterol concentration in the sample.
APPLICATIONS Direct Assays: HDL and LDL/VLDL cholesterol in serum samples. Pharmacology: evaluation of drugs on cholesterol metabolism.
KEY FEATURES Sensitive and accurate. Linear detection range in 96-well plate: 1-100 mg/dL cholesterol for colorimetric assays and 0.2 to 10 mg/dL for fluorimetric assays. Convenient. Room temperature assay. No 37°C heater is needed.
STORAGE CONDITIONS Store PBS and Precipitation Reagent at room temperature and the rest reagents at -20°C. Shelf life of at least 6 months.
其他 :编号：E2HL-048, E2HL-100
测试：48T, 100T