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产品说明 本公司研制的ADP检测试剂盒可以快速检测细胞ADP含量。检测共分两步，首先用工作试剂将细胞溶解，释放出ATP和ADP。在荧光素酶的作用下，ATP可与D-虫荧光素快速反应并发光，其光强度可以作为细胞间ATP浓度的直接衡量标准。然后，ADP通过酶化反应转化为ATP。而新生成的ATP再次以同样方式与D-虫荧光素发生反应。本产品通过试剂配方的优化，大大提高了光信号的稳定性。检测中，初次ATP检测所产生的光量将始终保持稳定。因此，第二次光强度检测可表征样本中总的ADP与ATP浓度。该检测无放射性，可用微板进行操作，试剂适用于所有培养液。在利用96或384孔板进行高通量筛选时，试剂适用于所有液体操作系统。
产品特点 安全：检测无放射性。测量敏感、精确：可测定0.1μM的ADP。操作简便：“混合-反应-测量”型检测，无需清洗及转移试剂操作。高通量：在96孔板和384孔板检测中，Z’因子大于0.5。通过高通量液体操作系统，每天可检测上千份样本。
产品用途 可用于细胞中或其他生物样本中ADP含量的检测。
储存条件 所有试剂于-20℃下保存。冰运。
DEscriptION Our ADP Assay Kit provides a rapid method to measure ADP levels. The assay involves two steps. In the first step, the working reagent lyses cells to release ATP and ADP. In the presence of luciferase, ATP immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration. In the second step, the ADP is converted to ATP through an enzyme reaction. This newly formed ATP then reacts with the D-luciferin as in the first step. Due to a special formulation of the reagent system which greatly stabilizes the light signal generated by the luciferase reaction, the luminescence from the initial ATP measurement remains stable throughout this assay. Therefore, the second light intensity measured represents the total ADP and ATP concentration in the sample. This non-radioactive, homogeneous cell-based assay is performed in microplates. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates.
KEY FEATURES Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.1 μM ADP can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z’ factors of 0.5 and above are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
APPLICATIONS ADP determination in cells and other biological samples.
STORAGE CONDITIONS store all reagents at -20°C. This product is shipped on ice. Shelf life of at least 6 months.
其他 :编号：EADP-048，EADP-100
测试：48T,100T