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测定方法 快速生物发光法测定组织或细胞中ADP：ATP的比例。检测时间：20分钟。运输：冷运。储存：-20°C。
在ADP／ATP比值描述变化来区分细胞死亡和生存模式。ATP和ADP水平升高水平下降意味着细胞增殖。相反，降低水平的ATP和ADP的水平增加是细胞凋亡或坏死的细胞中ATP和ADP在增加减少更明显坏死和凋亡。我们的enzylighttm ADP／ATP比值检测试剂盒提供了一种快速测定凋亡细胞的筛选ADP和ATP水平的方法，在哺乳动物细胞坏死和细胞增殖。该方法包括两个步骤。在步中，工作试剂裂解细胞释放ATP和ADP。在酶的作用下，ATP立即反应底物D-荧光素发光。光照强度是直接用来测量细胞内ATP浓度。第二步，ADP通过酶反应转化为ATP。这个新成立的ATP反应与荧光素作为步。由于一个试剂系统，大大提高了荧光素酶反应产生的光信号的特殊配方，从最初的ATP测量发光在本法保持稳定。因此，第二光强度测量的是样品中的总的ADP和ATP浓度。这种非放射性的，均匀的细胞检测在微孔板中进行。该试剂是兼容所有的培养基以及所有液体处理系统的高通量筛选应用96孔和384孔板。
Description Changes in the ADP/ATP ratio have been used to differentiate modes of cell death and viability. Increased levels of ATP and decreased levels of ADP signify proliferating cells. Conversely, decreased levels of ATP and increased levels of ADP represent apoptotic or necrotic cells wher the decrease in ATP and increase in ADP is much more pronounced in necrosis versus apoptosis. our EnzyLightTM ADP/ATP Ratio Assay Kit provides a rapid method to measure ADP and ATP levels for the screening of apoptosis, necrosis and cell proliferation in mammalian cells. The assay involves two steps. In the first step, the working reagent lyses cells to release ATP and ADP. In the presence of luciferase, ATP immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration.In the second step, the ADP is converted to ATP through an enzyme reaction. This newly formed ATP then reacts with the D-luciferin as in the first step. Due to a special formulation of the reagent system which greatly stabilizes the light signal generated by the luciferase reaction, the luminescence from the initial ATP measurement remains stable throughout this assay. Therefore, the second light intensity measured represents the total ADP and ATP concentration in the sample.This non-radioactive, homogeneous cell-based assay is performed in microplates. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates.
Key features Safe. Non-radioactive assay.Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.Robust and amenable to HTS: Z’ factors of 0.5 and above are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Applications Apoptosis and Necrosis determination in cells.Cell proliferation: effects of cytokines, growth factor, nutrients.Drug discovery: high-throughput screening for anticancer drugs.
Storage conditions: store all reagents at -20°C. This product is shipped on dry ice. Shelf life of at least 6 months.
其他 :编号：ELDT-048，ELDT-100
测试：48T,100T