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产品说明 三磷酸腺苷(ATP)是细胞代谢的化学能量，常被称为细胞的“能源货币”。ATP只在活细胞进行光合作用和呼吸作用时产生，在细胞生命活动包括生物合成反应、细胞运动和细胞分裂过程中消耗。它是细胞活动的重要指标，并且在研究和药物研发中已被用作一种细胞活性和细胞毒性的检测方法。本公司生产的ATP检测试剂盒为测量细胞内的ATP提供了一种快速的方法。用专门的工作试剂将细胞溶解，释放出ATP，ATP在荧光素酶的作用下，立即与底物D-荧光素反应产生光。光的强度可直接用来测量细胞内ATP浓度。这种非放射性、均相的细胞检测在微孔板中进行。该试剂适用于所有培养基和高通量药物筛选中应用的96孔板、384孔板和液体加样设备。
产品特点 安全: 无放射性检测。灵敏、准确: 检测 0.1 mM ATP 或 40个细胞。均相、方便: 混合-检测型”的均相检测方式。不涉及清洗和试剂转移的步骤。高通量: 在96孔板和384孔板中常观察到Z’ 因子>0.5。采用自动化的液体加样设备，每天可处理上千上万个样本。
产品用途 细胞和其他生物样品中ATP的测定。
试剂盒规格 10 mL 缓冲液, 120 uL 底物试剂, 5 mL ATP酶, 100 uL 3 mM ATP 标准品。
储藏条件 冰上运输，-20°C 保存。保质期6个月。
Description Adenosine 5-triphosphate (ATP) is the chemical energy for cellular metabolism and is often referred to as “energy currency" of the cell. ATP is produced only in living cells during photosynthesis and cellular respiration and consumed in cellular processes including biosynthetic reactions, motility and cell division. It is a key indicator of cellular activity and has been utilized as a measure of cell viability and cytotoxicity in research and drug discovery. Our ATP Assay Kit provides a rapid method to measure intracellular ATP. The single working reagent lyses cells to release ATP, which, in the presence of luciferase, immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration. This non-radioactive, homogeneous cell-based assay is performed in microplates. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates.
Key features Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.1 mM ATP or 40 cells can be quantified.Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.Robust and amenable to HTS: Z’ factors of > 0.5 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Applications ATP determination in cells and other biological samples.
Kit Contents 10 mL Assay Buffer, 120 uL Substrate, 5 uL ATP Enzyme, 100 uL Standard.
Storage conditions store all reagents at -20°C. This kit is shipped on ice. Shelf life of at least 6 months.
其他 :编号：EATP-100
测试：100T